Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• –liquid exposure; dosimetry; lung cells in vitro; silver nanoparticles; toxicity; Introduction Silver possesses antiseptic and germicidal properties [1]. These effects are enhanced in combination with the possibilities of nanotechnology, when silver is manufactured as particles at the nanoscale. Defined

• breaking of DNA strands and covalent DNA modifications [10]. Hence, Ag NPs have been shown to cause significant DNA damage in human lung cells in vitro [25][27] suggesting a potential genotoxic mechanism. Despite this, the specific interaction of Ag NPs with cells still remains unclear. Ag NPs released

• ISDD model it was possible to compare dose effects in two distinct exposure scenarios. Currently three different strategies are used to investigate possible effects of inhaled Ag NPs. Most commonly Ag NPs are applied in dispersion onto different lung cells in vitro [22][30][37][50][51], animal models

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

• Nadine Haberl,
• Stephanie Hirn,
• Alexander Wenk,
• Jörg Diendorf,
• Matthias Epple,
• Blair D. Johnston,
• Fritz Krombach,
• Wolfgang G. Kreyling and
• Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

• be able to cross the air-blood-barrier (ABB), towards circulation, and accumulate in secondary organs [16][17]. Some in vitro studies have demonstrated toxic effects of AgNP on lung cells: In vitro incubation of a rat alveolar macrophage cell line with AgNP induced a concentration- as well as a size